The Characteristics of an Ovine Lymphoid Cell-Line sensitive to Vaccinal Infectious Bursal Disease Virus Strain

Authors

  • Abdolhamid Shoushtari Management of Research and Diagnosis of Poultry Diseases, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
  • Ali Eshaghi Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran
  • Effat Parmour Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran
  • Hosein Goudarzi 5. Research and diagnosis of poultry diseases management of Razi Vaccine and Serum Research Institute, Karaj, Iran.
  • Mohammad Hasan Hablolvarid Department of Pathology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization, Karaj, Iran
  • Mohsen Lotfi Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran
  • Morteza Kamalzadeh Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran
  • Nadieh Shiri Department of Quality Control, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran
  • Saeid Amanpour Center of Cancer Biology Research, Tehran University of Medical Sciences, Tehran, Iran
  • Sakineh Zandieh Department of Genetics, Faculty of Sciences, Islamic Azad University, Ahar, Iran
  • Shahin Masoudi Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Research Institute, Agricultural Research, Education, and Extension Organization,Karaj, Iran
Abstract:

Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.

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Journal title

volume 72  issue 3

pages  173- 179

publication date 2017-09-01

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